Nuclear Physics and Atomic Energy

ядерна ф≥зика та енергетика
Nuclear Physics and Atomic Energy

  ISSN: 1818-331X (Print), 2074-0565 (Online)
  Publisher: Institute for Nuclear Research of the National Academy of Sciences of Ukraine
  Languages: Ukrainian, English, Russian
  Periodicity: 4 times per year

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Nucl. Phys. At. Energy 2016, volume 17, issue 3, pages 276-286.
Section: Radiobiology and Radioecology.
Received: 18.03.2016; Accepted: 29.06.2016; Published online: 13.12.2016.
PDF Full text (ua)
https://doi.org/10.15407/jnpae2016.03.276

Combined effects of thermal neutrons, gamma rays, red band light and neutron capture agents to the morphological indicators of cells line L929 in vitro

H. Yo. Lavrenchuk1,*, Yu. B. Shevchenko2, V. V. Tryshyn2, V. A. Libman2, I. V. Kozlovska1,
D. D. Gapeenko1, Ya. V. Pshenychnyi1, V. M. Venedyktov2, Yu. S. Ivanov3


1 SI "National Research Center for Radiation Medicine of NAMS of Ukraine", Kyiv, Ukraine
2 Institute for Nuclear Research, National Academy of Sciences of Ukraine, Kyiv, Ukraine
3 Main Astronomical Observatory, National Academy of Sciences of Ukraine, Kyiv, Ukraine


*Corresponding author. E-mail address: hl20071956@ukr.net

Abstract: Combined effect of neutron, gamma and light (red band) irradiation to morphological changes in the cell culture was investigated. It was found that in the presence of photosensitizing agents with neutron capture properties with molecular structure and also magnetically driven nano-complexes of both combined neutrons and red light causes around 93 % cell death of proliferating cells in vitro.

Keywords: culture proliferating cells, thermal neutrons, the red light range, survival, apoptosis.

References:

1. E.L. Kuplennikov, A.N. Dovbnya, Yu.N. Telegin et al. Neutron beam therapy: review (Kharkov: KIPT, 2011) 31 p. (Rus) IAEA

2. S.N. Navruzov, Zh.N. Kakhkhorov, N.Kh. Khodzhaeva. Archives of clinical and experimental medicine 23(1) (2014) 20. (Rus)

3. G.I. Borisov. Theoretical and experimental physical methods of neutron-capture therapy. Phys. Part. Nucl. 42(5) (2011) 713. (Rus) https://doi.org/10.1134/S1063779611050029

4. —. Salt, A.J. Lennox, M. Takagaki et al. Boron and gadolinium neutron capture therapy. Russian Chemical Bulletin 53(9) (2004) 1871. https://doi.org/10.1007/s11172-005-0045-6

5. A.P. Baranov, S.N. Koryakin, S.E. Ul'yanenko et al. Rossiysky Bioterapevtichesky Zhurnal 6(1) (2007) 80. (Rus)

6. N.F. Gamaleya, V.V. Kutsenok. Doctor 4 (2003) 28. (Rus)

7. S.V. Moskvin, V.A. Buylin. Basics of laser therapy (Tver: Triada, 2006) 256 p. (Rus)

8. A.F. Tsyb, M.A. Kaplan. Rossiyskie Meditsinskie Vesti 3 (2002) 19. (Rus)

9. V.E. Olyushin, A.V. Komfort, A.Yu. Ulitin. Rossiysky Bioterapevtichesky Zhurnal 6(1) (2007) 23. (Rus)

10. R.G. Nikitina, V.P. Mardynskaya, T.G. Morozova et al. Byulleten' Eksperimental'noj Biologii i Meditsiny 148(8) (2009) 209. (Rus)

11. R.P. Evstigneeva, A.V. Zaitsev, V.N. Luzgina et al. Carboranylporphyrins for boron neutron capture therapy of cancer. Current Medicinal Chemistry - Anticancer Agents 3(6) (2003) 383. https://doi.org/10.2174/1568011033482260

12. V.A. Ol'shevskaya, A.V. Zaitsev, V.N. Luzgina et al. Novel boron containing derivatives of 5,10,15,20-tetraphenylporphyrin: synthesis and toxicity for drug-resistant tumor cells. Bioorganic and Medicinal Chemistry 14(1) (2006) 109. https://doi.org/10.1016/j.bmc.2005.07.067

13. M.V. Lisovskij, A.P. Zhdanov, K.Yu. Zhizhin, N.T. Kuznetsov. Rossiysky Bioterapevtichesky Zhurnal 6(1) (2007) 48. (Rus)

14. M.A. Kaplan, R.G. Nikitina, V.A. Ol'shevskaya et al. Rossiysky Bioterapevtichesky Zhurnal 6(1) (2007) 16. (Rus)

15. Yu.V. Kul'velis, V.A. Trunov, V.T. Lebedev et al. Poverkhnost'. Rentgenovskie, Sinkhrotronnye i Nejtronnye Issledovaniya 5 (2009) 55. (Rus)

16. V.A. Ol'shevskaya, A.V. Zajtsev, E.G. Kononova et al. Doklady Akademii Nauk 414(2) (2007) 199. (Rus)

17. Yu.V. Kul'velis, V.A. Trunov, V.T. Lebedev et al. Fizika Tverdogo Tela 52(5) (2010) 974. (Rus) Article

18. A.V. Knyazev, E.N. Bulanov, D.Ya. Alejnik et al. Vestnik Nizhegorodskogo Universiteta im. N. I. Lobachevskogo 5(1) (2012) 88. (Rus) Article

19. V.N. Kulakov, N.L. Shimanovskij, V.O. Panov et al. Rossiysky Bioterapevtichesky Zhurnal 9(3) (2010) 15. (Rus)

20. N.V. Klassen, V.V. Kedrov, N.P. Kobelev et al. Rossiysky Bioterapevtichesky Zhurnal 6(1) (2007) 75. (Rus)

21. L-929 (NCTC-clone 929, Clone of strain L) (Connective tissue, mouse) (21 December 2009). http://www.viromed.com/services/product/l929.htm

22. Research of apoptosis in radiation immunology: guidance (SI "Scientific Center for Radiation Medicine, Academy of Medical Sciences of Ukraine") (Kyiv, 2010) 27 p. (Ukr)

23. S.N. Lapach, A.V. Chubenko, P.N. Babich. Statistical methods in biomedical research using Excel. 2nd ed.( Kyiv: MORION, 2001) 408 p. (Rus) Book

24. H.Yo. Lavrenchuk. Survival and proliferative activity of L-cells exposure to neutrons of different energy. J. Toxicol. Abstr. 10(2) (1987) 5.