Nuclear Physics and Atomic Energy


Nuclear Physics and Atomic Energy

  ISSN: 1818-331X (Print), 2074-0565 (Online)
  Publisher: Institute for Nuclear Research of the National Academy of Sciences of Ukraine
  Languages: Ukrainian, English, Russian
  Periodicity: 4 times per year

  Open access peer reviewed journal


 Home page   About 
Nucl. Phys. At. Energy 2017, volume 18, issue 1, pages 72-80.
Section: Radiobiology and Radioecology.
Received: 15.11.2016; Accepted: 15.06.2017; Published online: 7.08.2017.
PDF Full Text (ua)
https://doi.org/

Radial growth and activity of antioxidant enzymes in the three post-radiation Cladosporium cladosporioides generations

A. V. Tugay1, T. I. Tugay1,*, V. A. Zheltonozhsky2, M. V. Zheltonozhskaya2, L. V. Sadovnikov2, G. V. Ponomarenko1, . B. Polischuk3

1 Zabolotny Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, Kyiv, Ukraine
2 Institute for Nuclear Research, National Academy of Sciences of Ukraine, Kyiv, Ukraine
3 National Technical University of Ukraine "Igor Sikorsky Kyiv Polytechnic Institute", Kyiv, Ukraine


*Corresponding author. E-mail address: tatyanatugay2@gmail.com.

Abstract: Radial growth rate and activity of antioxidant enzymes - superoxide dismutase (SOD), catalase and peroxidase - in three post-radiation generations of two strains of Cladosporium cladosporioides: control and with radioadaptive properties were investigated. It was established that hormesis effect by the increase rate of radial growth is more pronounced in post-radiation generations of the control strain than in the generations of strain with radioadaptive properties. However, in enzymatic antioxidant defense system significant hormesis effect is revealed only in three post-radiation generations strain with radioadaptive properties, which appears in the increase up to 2 times in SOD activity and the peroxidase activity up to 4 times.

Keywords: chronic radiation, post-radiation generation of Cladosporium cladosporioides, adaptation, antioxidant enzymes.

References:

1. T.I. Tugay, N.N. Zhdanova, T.I. Retchits et al., Influence of low level ionizing irradiation on spread of radiotropism among fungi, Zbirnyk Naukovykh Prats' Instytutu Yadernykh Doslidzhen' 10, No. 2 (2003) 72 - 79. (Rus)

2. T.I. Tugay, N.N. Zhdanova, V.A. Zheltonozhsky et al., The influence of ionizing radiation on spore germination and emergent hyphal growth response reactions of microfungi, Mycologia 4 (2006) 521 - 527. http://www.jstor.org/stable/20444733

3. T.I. Tugay, N.N. Zhdanova, V.A. Zheltonozhsky et al., Effects of ionizing radiation on the antioxidant system of microscopic fungi with radioadaptive properties found in the Chernobyl exclusion zone, Health Physics - Radiation Safety Journal 101, No. 4 (2011) 375 - 382. http://doi.org/10.1097/HP.0b013e3181f56bf8

4. N.N. Zhdanova, T.I. Tugay, J. Dighton et al., Ionizing radiation attracts soil fungi, Mycol. Res. 108, No. 9 (2004) 1089 - 1096. https://doi.org/10.1017/S0953756204000966

5. N.N. Zhdanova, V.A. Zakharchenko, A.I. Vasilevskaya et al., Mycobiota Ukrainian Polesie. The Consequences of the Chernobyl Accident, Kyiv: Naukova Dumka (2013), 383 p. (Rus)

6. N.N. Zhdanova, V.A. Zakharchenko, T.I. Tugay et al., Fungi lesion of inner locations object shelter, Problemy Bezpeky Atomnykh Elektrostantsii i Chornobylya 3, p. 1 (2005) 78 - 86. (Rus) http://mntc.smn.com.ua/downloads/2005_031/c78.pdf

7. G.A. Kochkina, T.G. Mirchink, P.A. Kozhevin et al., Radial growth rate of fungal colonies in respect of their ecology, Mikrobiologiia 5 (1978) 964 - 965. (Rus)

8. V.A. Kostyuk, A.I. Potapovich, Zh.V. Kovaleva, Voprosy Meditsinskoj Khimii 2 (1990) 88 - 91. (Rus)

9. M.A. Koroliuk, L.I. Ivanova, I.G. Maiorova, V.E. Tokarev, Methods of catalase activity determining, Laboratornoe Delo 1 (1988) 16 - 19. (Rus)

10. A. Thiyagarajan, K. Saravanakumar, V. Kaviyarasan, Optimizaton of extracellular peroxidase production from Coprinus sp., In. J. of Science and Tech. 7 (2008) 1 - 5. http://www.indjst.org/index.php/indjst/article/view/29603/25603

11. M.M. Bredford, A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein - Dye Binding, Analytical Biochemistry 72 (1976) 248 - 254. https://doi.org/10.1016/0003-2697(76)90527-3

12. J. Garnier-Laplacea, F. Alonzob, C. Adam-Guillerminb, Establishing relationships between environmental exposures to radionuclides and the consequences for wildlife: inferences and weight of evidence, ICRP (2013) 295 - 303. https://doi.org/10.1177/0146645315572311

13. P.M. Plonka, . Grabacka, Melanin synthesis in microorganisms - biotechnological and medical aspects, Acta Biochim Pol. 53, No. 3 (2006) 429 - 443. http://www.actabp.pl/pdf/3_2006/429s.pdf

14. J.P. Latge, H. Bouziane, M. Diaquin, Ultrastructure and composition of the conidial wall of Cladosporium cladosporioides, Can. J. Microbiol. 34, No. 12 (1988) 1325 - 1329. https://doi.org/10.1139/m88-232

15. T.I. Tugay, A.V. Tugay, Patent for Utility Model No. 71518 Ukraine, 12 1/16. Micromycetes screening method for producing melanin pigments with high antioxidant activity; the applicant and the owner of the Zabolotny Institute of Microbiology and Virology of NAS of Ukraine, No. u 201203395; applicant. 21.03.2012; published 10.07.2012, bulletin No. 13. (Ukr)

16. T.I. Tugay, A.V. Tugay, V.A. Zheltonozhsky et al., Patent for Utility Model No. 73184 Ukraine, 12 N 1/14. A method for producing melanin pigments; the applicant and the owner of the Zabolotny Institute of Microbiology and Virology of NAS of Ukraine, No. u 201203789; applicant. 28.03.2012; published 10.09.2012, bulletin. No. 7. (Ukr)

17. A.A. Mir, S-Y. Park, Md.A. Sadat et al., Systematic characterization of the peroxidase gene family provides new insights into fungal pathogenicity in Magnaporthe oryzae, Scientific Reports 5 (2015) 11831. http://doi.org/10.1038/srep11831

18. A. Tugay, T. Tugay, D. Lukashov, Influence of low chronic exposure on physiological and biochemical properties of three irradiated generations Aspergillus versicolor, Visnyk Kyyivs'kogo Natsional'nogo Universytetu imeni Tarasa Shevchenka. Seriya "Biologiya" 70, No. 2 (2015) 77 - 81. (Ukr) http://dx.doi.org/10.17721/1728_2748.2015.70.77-81