Nuclear Physics and Atomic Energy

ядерна ф≥зика та енергетика
Nuclear Physics and Atomic Energy

  ISSN: 1818-331X (Print), 2074-0565 (Online)
  Publisher: Institute for Nuclear Research of the National Academy of Sciences of Ukraine
  Languages: Ukrainian, English, Russian
  Periodicity: 4 times per year

  Open access peer reviewed journal

 Home page   About 
Nucl. Phys. At. Energy 2018, volume 19, issue 2, pages 145-149.
Section: Radiobiology and Radioecology.
Received: 12.02.2018; Accepted: 18.06.2018; Published online: 02.08.2018.
PDF Full text (ua)

Relative radiosensitivity of Arabidopsis Thaliana Atmsh2 SALK_002708 mutant in the sublethal dose range of radiation

S. V. Litvinov*, N. M. Rashydov

Institute of Cell Biology and Genetic Engineering, National Academy of Sciences of Ukraine, Kyiv, Ukraine

*Corresponding author. E-mail address:

Abstract: Effective repair of radiation-induced DNA lesions is an important factor in the radioresistance of plants. Taking into account the role of the DNA mismatch repair (MMR) in the systemic reaction of plants to the action of sublethal doses of ionizing radiation has not yet been sufficiently clear enough. The study of the effect of low LET ionizing radiation in doses up to 21 Gy had been performed, including measurement of the biomass accumulation in Arabidopsis thaliana Atmsh2-/- plants, defective in one of the key components of MMR-repair, MSH2 protein. It has been established that the relative radiosensitivity of Atmsh2 SALK_002708 mutants to the action of sublethal doses of radiation depends on the dose and mode of irradiation. Changes in the radiosensitivity of mutant plants at different doses and due to the fractionation of the dose can be related to the radiation-induced transcriptional response of genes, coding DNA repair proteins, which compensate the lack of MMR-repair or along with MSH2 participate in the mutagenic pathways of DNA repair.

Keywords: ionizing irradiation, sublethal doses, radiosensitivity, DNA mismatch repair, MSH2.


1. D.M. Grodzinskij. Radiobiology of Plants (Kyiv: Naukova dumka, 1989) 384 p. (Rus)

2. S.V. Litvinov. Effects of chronic exposure of seeds and seedlings of Arabidopsis thaliana by low doses of γ-radiation on plant growth and development. Yaderna Fizyka ta Energetyka (Nucl. Phys. At. Energy) 15(4) (2014) 406. (Rus)

3. W.D. Bradford et al. An Inexpensive Gel Electrophoresis-Based Polymerase Chain Reaction Method for Quantifying mRNA Levels. Cell Biology Education 4(2) (2005) 157.

4. A. Depeiges et al. A new transgene assay to study microsatellite instability in wild-type and mismatch-repair defective plant progenies. Plant Science 168 (2005) 939.

5. S. Litvinov, N. Rashydov. The transcriptional response of Arabidopsis thaliana L. AtKu70, At-RAD51 and AtRad1 genes to X-rays. Journal of Agricultural Science and Technology A 7(1) (2017) 52.

6. N. Tuteja et al. Molecular mechanisms of DNA damage and repair: progress in plants. Crit. Rev. Biochem. and Mol. Biol. 36(4) (2001) 337.

7. W.M. Waterworth et al. Repairing breaks in the plant genome: the importance of keeping it together. New Phytologist 192(4) (2011) 805.

8. K.O. Yoshiyama, K. Sakaguchi, S. Kimura. DNA Damage Response in Plants: Conserved and Variable Response Compared to Animals. Biology 2(4) (2013) 1338.