Nuclear Physics and Atomic Energy


Nuclear Physics and Atomic Energy

  ISSN: 1818-331X (Print), 2074-0565 (Online)
  Publisher: Institute for Nuclear Research of the National Academy of Sciences of Ukraine
  Languages: Ukrainian, English, Russian
  Periodicity: 4 times per year

  Open access peer reviewed journal


 Home page   About 
Nucl. Phys. At. Energy 2019, volume 20, issue 3, pages 271-277.
Section: Radiobiology and Radioecology.
Received: 04.04.2019; Accepted: 11.07.2019; Published online: 30.11.2019.
PDF Full text (ru)
https://doi.org/10.15407/jnpae2019.03.271

Recovery of the yeast cells from radiation injuries by means of the magnetic isotopes: New trend in anti-radiation biomedicine

L. V. Avdeeva1, T. A. Evstyukhina2, V. K. Koltover1,*, V. G. Korolev2, Y. A. Kutlakhmedov3

1 Institute of Problems of Chemical Physics, Russian Academy of Sciences, Chernogolovka, Moscow Region, Russia
2 Petersburg Institute of Nuclear Physics, NRC Kurchatov Institute, Gatchina, Leningrad Region, Russia
3 Institute of Cell Biology and Genetic Engineering, National Academy of Sciences of Ukraine, Kyiv, Ukraine


*Corresponding author. E-mail address: koltover@icp.ac.ru;

Abstract: Herein we present the results of studying the effects of different isotopes of magnesium, magnetic 25Mg and nonmagnetic 24Mg, upon the post-radiation recovery of yeast cells, S. cerevisiae, irradiated by short-wave UV light (240 - 260 nm) or ionizing radiation (300 Gy). The recovery process of the cells enriched with the magnetic 25Mg proceeds two times faster than the post-radiation recovery of the cells, enriched with the nonmagnetic 24Mg. After gamma-irradiation, the fraction of the irreversible damages in the cells enriched with 25Mg was 50 - 60 % less than in the cells enriched with 24Mg. Thus, the magnetic isotope effect has been detected, i.e. the acceleration of post-radiation recovery of the cells by the magnetic isotopes nuclear spin of magnesium (nuclear spin catalysis). Obtained results demonsrate the fundamental possibility of creating new radioprotectors and radiomitigators based on stable magnetic isotopes.

Keywords: post-radiation recovery, magnetic-isotope effect, nuclear spin catalysis, radioprotectors, radiomitigators, yeast cells, magnesium, reliability, robustness.

References:

1. Y.A. Kutlakhmedov, V.I. Korogodin, V.K. Koltover. Bases of Radioecolgy (Kyiv: High School, 2003) 323 p. (Ukr)

2. P. Bradford. The nuclear landscape. Nature 483 (2012) 151. https://doi.org/10.1038/483151a

3. J.C. Livesey, D.J. Reed, L.F. Adamson. Radiation-Protective Drugs and their Reaction Mechanisms (Park Ridge: Noyes Publications, 1985) 146 p. Google Books

4. V.K. Koltover, V.G. Korolev, Y.A. Kutlakhmedov. Antioxidant prophylaxis of radiation stress. In: Ionizing Radiation: Applications, Sources and Biological Effects (New York: Nova Science Publ., 2013) p. 117. https://doi.org/10.1016/j.freeradbiomed.2012.10.262

5. M.V. Vasin. The classification of radiation protective agents as the reflection of the present state and development perspective of current radiation pharmacology. Radiation Biology. Radioecology 53 (2013) 459. (Rus)

6. L.M. Rozhdestvensky. Actual problems of searching and studying radiation countermeasures. Radiation Biology. Radioecology 53 (2013) 513. (Rus)

7. A.N. Grebenyuk et al. Radiomitigators: prospects for use in medical radiation protection. Military Medical Journal 335 (2014) 39. (Rus)

8. V.N. Bykov et al. Radioprotective and radiomitigative effects of BP-C2, a novel lignin-derived polyphenolic composition with ammonium molybdate, in two mouse strains exposed to total body irradiation. Inter. J. Radiation Biol. 94 (2018) 114. https://doi.org/10.1080/09553002.2018.1416204

9. D.M. Grant, R.K. Harris. Encyclopedia of Nuclear Magnetic Resonance (Chichester: Wiley, 1996) 826 p. Google Books

10. Y.B. Zeldovich, A.L. Buchachenko, E.L. Frankevich. Magnetic spin effects in chemistry and molecular physics. Sov. Phys. Usp. 155 (1988) 3. https://doi.org/10.1070/PU1988v031n05ABEH003544

11. A.L. Buchachenko, R.G. Lawler. New possibilities for magnetic control of chemical and biochemical reactions. Acc. Chem. Res. 50 (2017) 877. https://doi.org/10.1021/acs.accounts.6b00608

12. D.M. Grodzinsky et al. Effect of the magnetic isotope of magnesium-25 on the post-radiation recovery of Saccharomyces cerevisiae cells. Dopovidi NAN Ukrayiny 12 (2011) 153. (Rus)

13. D.M. Grodzinsky et al. Investigation of the influence of the magnesium isotope-25 on the post-radiation recovery of Saccharomyces cerevisiae cells. Naukovi pratsi Chornomorskoho derzhavnoho universytetu imeni Petra Mohyly. Ser. Tekhnohenna bezpeka 169 (2011) 76. (Rus) http://lib.chdu.edu.ua/pdf/naukpraci/technogen/2011/169-157-11.pdf

14. V.K. Koltover et al. Magnetic isotope effect of magnesium in the living cell. Doklady Biochem. Biophys. 442 (2012) 12. https://doi.org/10.1134/S1607672912010048

15. L.V. Avdeeva, V.K. Koltover. Nuclear spin catalysis in living nature. Moscow University Chemistry Bulletin 71 (2016) 160. https://doi.org/10.3103/S0027131416030020

16. V.K. Koltover. Nuclear spin catalysis: from physics of liquid matter to medical physics. J. Mol. Liquids 235 (2017) 44. https://doi.org/10.1016/j.molliq.2016.11.083

17. D.L. Nelson, M.M. Cox. Lehninger Principles of Biochemistry (New York: Freeman, 2008) 1294 p. Google Books

18. Y.V. Karyakin, I.I. Angelov. Pure Chemicals (Moskva: Khimia, 1974) 408 p. (Rus)

19. V.K. Karandashev et al. Use of the inductively coupled plasma mass spectrometry for element analysis of environmental objects. Inorg. Mater. 44 (2008) 1491. https://doi.org/10.1134/S0020168508140045

20. S.V. Kovaltsova et al. The geptrong pharmaceutical product increases efficiency of postreplication repair of permutation intermediates in yeast Saccharomyces cerevisiae. Russian J. Genetics 44 (2008) 1272. https://doi.org/10.1134/S1022795408110045

21. T.A. Evstiukhina et al. The role of remodeling complexes CHD1 and ISWI in spontaneous and UV-induced mutagenesis control in yeast Saccharomyces cerevisiae. Russian J. Genetics 53 (2017) 195. https://doi.org/10.1134/S1022795417010057

22. V.I. Korogodin. Problems of Postradiation Recovery (Moskva: Atomizdat, 1966) 391 p. (Rus)

23. Y.G. Kapultsevich. Quantitative Laws of Radiation Damages of Cells (Moskva: Atomizdat, 1978) 231 p. (Rus)

24. V.K. Koltover, Y.A. Kutlakhmedov, E.L. Afanaseva. Recovery of cells from radiation injuries with the aid of antioxidants and the reliability of biological systems. Doklady Biophysics (Doklady Akademii Nauk SSSR) 254 (1980) 159.

25. A. Novick, L. Szilard. Experiments on light-reactivation of ultraviolet inactivated bacteria. Proc. Natl. Acad. Sci. USA 35 (1949) 591. https://doi.org/10.1073/pnas.35.10.591

26. J.J. Vicente, L. Wordeman. Mitosis, microtubule dynamics and the evolution of kinesins. Exp. Cell. Res. 334 (2015) 61. https://doi.org/10.1016/j.yexcr.2015.02.010

27. V.K. Koltover et al. Magnetic isotope of magnesium accelerates ATP hydrolysis catalyzed by myosin. Biophysics (Eng. translation) 61 (2016) 200. https://doi.org/10.1134/S0006350916020068

28. V.K. Koltover, R.D. Labyntseva, S.O. Kosterin. Stable magnetic isotopes as modulators of ATPase activity of smooth muscle myosin. In: Myosin: Biosynthesis, Classes and Function (New York: Nova Science Publ., 2018) p. 135.

29. V.K. Koltover. Stable magnetic isotopes as a new trend in biomedicine. In: Biomedicine (Rijeka: InTech-Europe, 2012) p. 105.

30. V.D. Longo et al. Replicative and Chronological Aging in Saccharomyces cerevisiae. Cell Metabolism 16 (2012) 18. https://doi.org/10.1016/j.cmet.2012.06.002