Nuclear Physics and Atomic Energy

ядерна ф≥зика та енергетика
Nuclear Physics and Atomic Energy

  ISSN: 1818-331X (Print), 2074-0565 (Online)
  Publisher: Institute for Nuclear Research of the National Academy of Sciences of Ukraine
  Languages: Ukrainian, English
  Periodicity: 4 times per year

  Open access peer reviewed journal


 Home page   About 
Nucl. Phys. At. Energy 2021, volume 22, issue 3, pages 300-307.
Section: Radiobiology and Radioecology.
Received: 17.11.2021; Accepted: 22.12.2021; Published online: 22.02.2022.
PDF Full text (ua)
https://doi.org/10.15407/jnpae2021.03.300

Chromosome aberrations in human lymphocytes due to external and/or internal irradiation of blood samples by 137Cs in model experiments in vitro

V. A. Kurochkina*, L. K. Bezdrobna, T. V. Tsyhanok, M. V. Strilchuk, I. A. Maliuk

Institute for Nuclear Research, National Academy of Sciences of Ukraine, Kyiv, Ukraine

*Corresponding author. E-mail address: knitel@ukr.net

Abstract: The linear dependence of the yield of unstable chromosomal aberrations in human lymphocytes on the dose of prolonged external and/or internal irradiation of peripheral blood samples by 137Cs in the dose range of 0.09 - 0.54 Gy in vitro is presented. The higher cytogenetic efficiency of internal 137Cs irradiation is shown in comparison with external. It is shown that the cytogenetic efficiency of 137Cs in blood contamination is caused by influence on cells not only in the mitotic rest phase but during proliferation, within intracellular radioactivity through their cultivation. It is proposed to use the presented dose dependences of the yield of chromosomal aberrations in lymphocytes in vitro for human biological dosimetry during radiation accidents associated with prolonged external exposure or 137Cs incorporation to the body as a supplement to the results of physical dosimetry.

Keywords: 137Cs external irradiation, internal irradiation, human blood lymphocytes, chromosomal aberrations, biological dosimetry.

References:

1. The Radiological Accident in Goiania (Vienna, IAEA, 1988) 157 p. https://www-pub.iaea.org/mtcd/publications/pdf/pub815_web.pdf

2. Environmental Consequences of the Chernobyl Accident and their Remediation: Twenty Years of Experience. Report of the Chernobyl Forum Expert Group "Environment" (Vienna, IAEA, 2006) p. 23. https://www-pub.iaea.org/mtcd/publications/pdf/pub1239_web.pdf

3. The Radiological Accident in Tammiku (Vienna, IAEA, 1998) 70 p. https://www-pub.iaea.org/MTCD/Publications/PDF/Pub1053_web.pdf

4. The Radiological Accident in Lilo (Vienna, IAEA, 2000) 120 p. https://www-pub.iaea.org/MTCD/Publications/PDF/Pub1097_web.pdf

5. L.A. Il'in et al. Early medical consequences of radiation accidents in the former USSR Territory. Meditsina Truda i Promyshlennaia Ekologiia 10 (2012) 6. (Rus) https://pubmed.ncbi.nlm.nih.gov/23210177/

6. A.T. Ramalho, A.C. Nascimento. The fate of chromosomal aberrations in 137Cs exposed individuals in the Goiânia radiation accident. Health Phys. 60(1) (1991) 67. https://doi.org/10.1097/00004032-199101000-00010

7. C. Lindholm et al. Biodosimetry after accidental radiation exposure by conventional chromosome analysis and FISH. Int. J. of Radiat. Biol. 70(6) (1996) 647. https://doi.org/10.1080/095530096144527

8. A. Wojcik et al. Cytogenetic damage in lymphocytes for the purpose of dose reconstruction: a review of three recent radiation accidents. Cytogenet. Genome Res. 104(1-4) (2004) 200. https://doi.org/10.1159/000077489

9. A. Giussani et al. Eurados review of retrospective dosimetry techniques for internal exposures to ionising radiation and their applications. Radiat. Environ. Biophys. 59 (2020) 357. https://doi.org/10.1007/s00411-020-00845-y

10. N. Vulpis, G. Scarpa. Induction of chromosome aberrations by 90Sr β-particles in cultured human lymphocytes. Mutation Research 163 (1986) 277. https://doi.org/10.1016/0027-5107(86)90026-6

11. D.P. Morrison et al. Tritium β-radiation induction of chromosomal damage: a calibration curve for low dose, low dose rate exposures of human cells to tritiated water (Vienna, IAEA) 6 p. https://inis.iaea.org/collection/NCLCollectionStore/_Public/30/020/30020399.pdf?r=1&r=1

12. E.M. de Oliveira et al. Evaluation of the effect of 90Sr beta-radiation on human blood cells by chromosome aberration and single cell gel electrophoresis (comet assay) analysis. Mutation Research 476 (2001) 109. https://doi.org/10.1016/S0027-5107(01)00100-2

13. S. Roch-Lefèvre et al. A mouse model of cytogenetic analysis to evaluate caesium137 radiation dose exposure and contamination level in lymphocytes. Radiat. Environ. Biophys. 55(1) (2016) 61. https://doi.org/10.1007/s00411-015-0620-7

14. Relative Biological Effectiveness (RBE), Quality Factor (Q), and Radiation Weighting Factor (wR). ICRP Publication 92. Ann. ICRP 33(4) (2003). https://www.icrp.org/publication.asp?id=ICRP%20Publication%2092

15. L.K. Bezdrobna et al. Simulation of conditions for external and internal exposure of human blood to low doses of 137Cs radionuclide in vitro to study its genotoxicity. Yaderna Fizyka ta Energetyka (Nucl. Phys. At. Energy) 21(2) (2020) 166. https://doi.org/10.15407/jnpae2020.02.166

16. Cytogenetic Dosimetry: Applications in Preparedness for and Response to Radiation Emergencies. Russian Edition (Vienna, IAEA, 2011) 229 p. https://www.iaea.org/ru/publications/10455/cytogenetic-dosimetry-applications-in-preparedness-for-and-response-to-radiation-emergencies

17. E.A. Ainsbury, D.C. Lloyd. Dose estimation software for radiation biodosimetry. Health Phys. 98(2) (2010) 290. https://doi.org/10.1097/01.HP.0000346305.84577.b4

18. A.V. Sevankayev, I.K. Khvostunov., V.I. Potebnya. Cytogenetic effects of low doses and dose rates during γ irradiation of human blood lymphocytes in vitro. Radiatsionnaya Biologiya i Radioekologiya 52 (2012) 11. (Rus)

19. L.A. Beaugé, R.A. Sjodin. Transport of caesium in frog muscle. J. Physiol. 194(1) (1968) 105. https://doi.org/10.1113/jphysiol.1968.sp008397

20. S. Saremi, M. Isaksson, K.C. Harding. Bio accumulation of radioactive caesium in marine mammals in the Baltic Sea Ц Reconstruction of a historical time series. Science of the Total Environment 631-632 (2018) 7. https://doi.org/10.1016/j.scitotenv.2018.02.282

21. C. Edwards. The selectivity of ion channels in nerve and muscle. Neuroscience 7 (1982) 1335. https://doi.org/10.1016/0306-4522(82)90249-4

22. A. Balasem et al. Radiation-induced chromosomal aberrations in simulated internal contamination with radioactive caesium. Radiation Protection Dosimetry 42(4) (1992) 323. https://doi.org/10.1093/oxfordjournals.rpd.a081319

23. E.A. Djomina. The radiosensitivity of chromosomes of lymphocytes of peripheral humans' blood and mitosis cycle. Problemy Bezpeky Atomnykh Elektrostantsiy i Chornobylya 3(2) (2005) 80. (Rus) http://dspace.nbuv.gov.ua/bitstream/handle/123456789/128009/11-Demina.pdf?sequence=1