Ядерна фізика та енергетика Nuclear Physics and Atomic Energy   ISSN: 1818-331X (Print), 2074-0565 (Online)   Publisher: Institute for Nuclear Research of the National Academy of Sciences of Ukraine   Languages: Ukrainian, English   Periodicity: 4 times per year   Open access peer reviewed journal

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J. V. Shylina^1,*, O. S. Molozhava², S. V. Litvinov¹, O. P. Dmitriev<sup>1

1</sup> Institute of Cell Biology and Genetic Engineering, National Academy of Sciences of Ukraine, Kyiv, Ukraine
² Educational and Scientific Centre "Institute of Biology and Medicine", Taras Shevchenko National University of Kyiv, Kyiv, Ukraine

^*Corresponding author. E-mail address: j.shilina@gmail.com

Abstract: The effect of chronic irradiation of Pseudomonas aeruginosa (P. aeruginosa) phytopathogenic strain IMV 9096 and saprophytic strain IMV 8614 at a dose rate of 0.19 µGy/s of γ-radiation in the dose field of ¹³⁷Cs on the immunomodulatory properties of their lipopolysaccharide (LPS) has been investigated. It was shown that in the wild-type seedlings of Arabidopsis thaliana (A. thaliana) Col-0 pre-treatment with LPS 9096, isolated from both irradiated and non-irradiated bacterial culture, caused an increased harmful effect 2.8 - 5.6 fold when plants were then infected with bacteria of this strain. Seedling damage was more pronounced with the use of LPS, isolated from bacteria exposed to chronic radiation. In seedlings of mutant jin1 with impaired jasmonate signaling pre-treatment of LPS 9096 caused attenuation of the damage at 20 - 45 % when infected with P. aeruginosa 9096. Pre-treatment of Arabidopsis seeds with bacterial LPS 8614, obtained from both irradiated and non-irradiated P. aeruginosa 8614 cultures, had a non-significant effect (± 15 % over control). In mutant plants, jin1 pre-treatment of seeds with LPS 8614 led to increased damage when infected with P. aeruginosa IMV 9096 at 30 - 60 %. It was found that chronic irradiation of bacteria changes the immunomodulatory properties of their LPS and the effect depends on the bacterial strain. This effect is mediated by jasmonate and salicylate signaling systems.

Keywords: ¹³⁷Cs, chronic irradiation, lipopolysaccharide, Arabidopsis thaliana, Pseudomonas aeruginosa.

1. M.T. Kingsley et al. The opsX locus of Xanthomonas campestris affects host range and biosynthesis of lipopolysaccharide and extracellular polysaccharide. J. Bacteriol. 175 (1993) 5839. https://doi.org/10.1128/jb.175.18.5839-5850.1993

2. J.V. Shilina et al. Induction of Arabidopsis thaliana Resistance to Pathogenic Bacteria by Lipopolysaccharide and Salicylic Acid. Cytol. Genet. 52(3) (2018) 169. https://doi.org/10.3103/S0095452718030118

3. Yu.V. Shilina et al. Evaluation of the effect of bacterial lipopolysaccharides on the resistance of Arabidopsis thaliana plants to phytopathogenic bacteria. Visnyk Ukrayinskoho Tovarystva Henetykiv i Selektsioneriv 13(1) (2015) 100. (Ukr) http://utgis.org.ua/journals/index.php/VisnykUTGiS/article/view/397

4. Yu.V. Shilina et. al. Immunomodulatory properties of bacterial lipopolysaccharides in Arabidopsis thaliana plants and their modification. Fiziolohiya Roslyn ta Henetyka 49(2) (2017) 121. (Ukr) https://doi.org/10.15407/frg2017.02.121

5. V.A. Sharpaty. Radiation Chemistry of Biopolymers (Moskva: GEOS, 2008) 250 p. (Rus) Google books

6. V.A. Sharpaty. Problems of radiation chemistry of polysaccharides. Radiatsionnaya Biologiya. Radioekologiya 1 (1999) 156. (Rus)

7. Y. Li et al. X-ray Irradiated Vaccine Confers Protection Against Pneumonia Caused by Pseudomonas Aeruginosa. Sci. Rep. 6 (2016) 18823. https://doi.org/10.1038/srep18823

8, L.D. Varbanets, G.M. Zdorovenko, Yu.A. Knirel. Methods for the Endotoxins Study (Kyiv: Naukova Dumka, 2006) 237 p. (Rus)

9. I.A. Dudka et al. Methods of Experimental Mycology (Kyiv: Naukova Dumka, 1982) 552 p. (Rus)

10. L.D. Varbanets. Endotoxins of gram-negative bacteria: structure and biological role. Mikrobiolohichnyy Zhurnal 56(3) (1994) 76. (Rus)

11. Yu.V. Yezepchuk. Pathogenicity as a Function of Biomolecules (Moskva: Meditsina, 1985) 240 p. (Rus)

12. C.R.H. Raetz, C. Whitfield. Lipopolysaccharide endotoxins. Annu. Rev. Biochem. 71 (2002) 635. https://doi.org/10.1146/annurev.biochem.71.110601.135414

13. V. Lai, L. Wang, P.R. Reeves. Escherichia coli clone Sonnei (Shigella sonnei) had a chromosomal O-antigen gene cluster prior to gaining its current plasmid-borne O-antigen genes. J. Bacteriol. 180(11) (1998) 2983. https://doi.org/10.1128/JB.180.11.2983-2986.1998

14. A.A. Hassan, C.P. Coutinho, I. Sá-Correia. Burkholderia cepacian Complex Species Differ in the Frequency of Variation of the Lipopolysaccharide O Antigen Expression During Cystic Fibrosis Chronic Respiratory Infection. Front. Cell. Infect. Microbiol. 9 (2019) 273. https://doi.org/10.3389/fcimb.2019.00273

15. M. Mancilla et al. Spontaneous Excision of the O polysaccharide wbkA Glycosyltranferase Gene is a Cause of Dissociation of Smooth to Rough Brucella Colonies. J. Bacteriol. 194(8) (2012) 1860. https://doi.org/10.1128/JB.06561-11

16. P.G. Nyholm et al. Conformation of the O-specific polysaccharide of Shigella dysenteriae type 1: molecular modeling shows a helical structure with efficient exposure of the antigenic determinant α-L-Rhap-(1→2)-α-D-Galp. Glycobiology 11(11) (2001) 945. https://doi.org/10.1093/glycob/11.11.945

17. M.S. Trent et al. PhoP/PhoQ-induced lipase (PagL) that catalyzes 3-O-deacylation of lipid a precursors in membranes of Salmonella typhimurium. J. Biol. Chem. 276(12) (2001) 9083. https://doi.org/10.1074/jbc.M010730200

18. A.B. Schromm et al. Biological activities of lipopolysaccharides are determined by the shape of their lipid A portion. Eur. J. Biochem. 267(7) (2000) 2008. https://doi.org/10.1046/j.1432-1327.2000.01204.x

19. U. Seydel et al. Intrinsic conformation of lipid A is responsible for agonistic and antagonistic activity. Eur. J. Biochem. 267(10) (2000) 3032. https://doi.org/10.1046/j.1432-1033.2000.01326.x

20. H. Mayer, R.M. Tharanotham, J. Weckesser. Analysis of lipopolysaccharides of gram-negative bacteria. Methods in Microbiology 18 (1985) 157. https://doi.org/10.1016/S0580-9517(08)70475-6

21. Yu.V. Shilina et al. The role of the optional component of the genome in adaptive modifications of bacterial lipopolysaccharides. In: Factors of Experimental Evolution of Organisms. Collection of Scientific Works. Vol. 11 (Kyiv: Logos, 2011) p. 180. (Ukr) http://utgis.org.ua/images/pdf/faktory/tom_11.pdf

22. L. Caarls et al. Assessing the role of ethylene response factor transcriptional repressors in salicylic acid-mediated suppression of jasmonic acid-responsive genes. Plant & Cell Physiol. 58(2) (2017) 266. https://doi.org/10.1093/pcp/pcw187

23. M. Naseem, M. Kunz, T. Dandekar. Probing the unknowns in cytokinin-mediated immune defense in Arabidopsis with systems biology approaches. Bioinform. Biol. Insights 8 (2014) 35. https://doi.org/10.4137/BBI.S13462

24. J.S. Thaler, P.T. Humphrey, N.K. Whiteman. Evolution of jasmonate and salicylate signal crosstalk. Trends in Plant Science 17(5) (2012) 260. https://doi.org/10.1016/j.tplants.2012.02.010

25. A. Koornneef, C.M.J. Pieterse. Cross talk in defense signaling. Plant Physiology 146(3) (2008) 839. https://doi.org/10.1104/pp.107.112029

26. G. Loake, M. Grant. Salicylic acid in plant defense – the players and protagonists. Curr. Opin. Plant Biol. 10(5) (2007) 466. https://doi.org/10.1016/j.pbi.2007.08.008

27. S.H. Spoel et al. NPR1 modulates cross-talk between salicylate- and jasmonate-dependent defense pathways through a novel function in the cytosol. The Plant Cell 15(3) (2003) 760. https://doi.org/10.1105/tpc.009159