Nuclear Physics and Atomic Energy

ßäåðíà ô³çèêà òà åíåðãåòèêà
Nuclear Physics and Atomic Energy

  ISSN: 1818-331X (Print), 2074-0565 (Online)
  Publisher: Institute for Nuclear Research of the National Academy of Sciences of Ukraine
  Languages: Ukrainian, English
  Periodicity: 4 times per year

  Open access peer reviewed journal


 Home page   About 
Nucl. Phys. At. Energy 2023, volume 24, issue 4, pages 376-381.
Section: Radiobiology and Radioecology.
Received: 30.10.2023; Accepted: 22.11.2023; Published online: 28.12.2023.
PDF Full text (ua)
https://doi.org/10.15407/jnpae2023.04.376

Estimation of the reserve capacity of Myodes glareolus after acute irradiation using hematological indicators

O. B. Ganzha*, V. V. Pavlovskyi

Institute for Nuclear Research, National Academy of Sciences of Ukraine, Kyiv, Ukraine

*Corresponding author. E-mail address: olganzha@ukr.net

Abstract: The problem of identifying the sensitivity of living organisms to ionizing irradiation remains relevant, considering the spread of anthropogenic environmental pollution. The study on the effect of single X-ray irradiation (1,5 Gy) on peripheral blood of bank voles (Myodes glareolus (Schreber, 1780)) captured within territories with background radiation level was conducted. Hematological indicators, characterizing the overall condition of performance of the body, were determined dynamically on the first and seventh days after exposure to detect both early changes and the rate of recovery processes. The patterns and features of the main components of leukocyte formula found in peripheral blood of irradiated animals are being discussed. Differences between irradiated and control mouse-like rodents are shown, using parameters of erythrocytes and leukocytes. The analysis of changes in the peripheral blood of irradiated bank voles indicates the high reserve capacity of the body, according to its ability to restore homeostasis.

Keywords: bank vole, X-ray irradiation, peripheral blood, hematological indicators.

References:

1. N.K. Rodionova et al. Influence of radiation conditions of the Chernobyl Exclusion Zone on the hematopoietic system of bank vole. Yaderna Fizyka ta Energetyka (Nucl. Phys. At. Energy) 20(1) (2019) 44. (Ukr) https://doi.org/10.15407/jnpae2019.01.044

2. A.I. Lypska et al. Estimation of status of small rodents' natural populations from the transformed ecosystems of the Chornobyl exclusion zone according to the complex of biological indicators. Yaderna Fizyka ta Energetyka (Nucl. Phys. At. Energy) 21(4) (2020) 328. (Ukr) https://doi.org/10.15407/jnpae2020.04.328

3. X.H. Li et al. Effects of Low-to-Moderate Doses of Gamma Radiation on Mouse Hematopoietic System. Radiat. Res. 190 (2018) 612. https://doi.org/10.1667/RR15087.1

4. J.G. Kiang et al. Female Mice are More Resistant to the Mixed-Field (67% Neutron + 33% Gamma) Radiation-Induced Injury in Bone Marrow and Small Intestine than Male Mice due to Sustained Increases in G-CSF and the Bcl-2/Bax Ratio and Lower miR-34a and MAPK Activation. Radiat. Res. 198 (2022) 120. https://doi.org/10.1667/RADE-21-00201.1

5. J.H. Ware et al. Effects of Proton Radiation Dose, Dose Rate and Dose Fractionation on Hematopoietic Cells in Mice. Radiat. Res. 174(3) (2010) 325. https://doi.org/10.1667/RR1979.1

6. R.E. Raskin, K.S. Latimer, H. Tvedten. Chapter 4. Leukocyte Disorders. In: M.D. Willard, H. Tvedten (Eds.) Small Animal Clinical Diagnosis by Laboratory Methods. 4th ed. (W.B. Saunders, 2004) p. 63. https://doi.org/10.1016/B0-72-168903-5/50008-2

7. O.S. Monastyrska. Clinical Laboratory Tests. M.B. Shehedin (Ed.) (Vinnytsia: Nova Knyha, 2007) 168 p. (Ukr)

8. Law of Ukraine No. 3447 IV "On the Protection of Animals from Cruelty". Vidomosti Verkhovnoyi Rady Ukrayiny 27 (2006) 230. (Ukr) https://zakon.rada.gov.ua/laws/show/3447-15#Text

9. H. Selye. Stress Without Distress. Transl. from Eng. by A.N. Luka, I.S. Khorol. E.M. Kreps (Ed.) (Moskva: Progress, 1979) 128 p. (Rus)

10. E.B. Grigorkina. Natural radioresistance as a criterion of species (as exemplified by large taxa of the order rodentia). Dokl. Biol. Sci. 385 (2002) 371. https://doi.org/10.1023/A:1019925204359

11. V.M. Zaporozhan et al. Morphology of Blood Cells of Laboratory Animals and Humans: Atlas (Odesa: Odesa State Medical University, 2002) 118 p. (Ukr) Atlas

12. T.A. Shiftan, J. Mendelsohn. The circulating "atypical" lymphocyte. Human Pathology 9(1) (1978) 51. https://doi.org/10.1016/S0046-8177(78)80007-0